Journal: Genome Biology

Article Title: Linked optical and gene expression profiling of single cells at high-throughput

doi: 10.1186/s13059-020-01958-9

Figure Lengend Snippet: A high-throughput platform for linked optical phenotype and gene expression of single cells. a Monodisperse droplet emulsions containing encapsulated poly-T mRNA capture beads and cells are input into a microfluidic device. Fluorescence signal from droplets is interrogated and used to selectively dispense a cell and bead to indexed locations on a nanowell array. b Each bead binds mRNA from cell lysate as well as a unique combination of poly-A barcode oligos denoted by nanowell coordinate. c UMI counts on each bead are collected through sequencing into an expression matrix for each cell. Nanowell coordinate is assigned based on the abundance of barcode oligos and paired with fluorescence data obtained during cell sorting, which enables downstream linked analyses such as dimensionality reduction visualizations of gene expression paired with optical phenotype

Article Snippet: Barcoded mRNA capture beads are purchased through ChemGenes (MACOSKO-2011-10) and have a structure previously reported [ ].

Techniques: High Throughput Screening Assay, Expressing, Fluorescence, Sequencing, FACS

Journal: Immunity

Article Title: CD4 + T cells re-wire granuloma cellularity and regulatory networks to promote immunomodulation following Mtb reinfection

doi: 10.1016/j.immuni.2024.08.002

Figure Lengend Snippet:

Article Snippet: Massively parallel scRNA-seq was performed using the Seq-Well S 3 platform, as previously described., Approximately 15,000-20,000 cells were loaded onto Seq-Well arrays equipped with uniquely barcoded mRNA capture beads (ChemGenes).

Techniques: Virus, Recombinant, Membrane, Infection, Software